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In comparability, Figure 3b reveals a PAS-stained histology of an uninfected porcine hoof fragment. During the experiment, the porcine hoof or human toenail fragments had been placed into 32-mm Petri dishes with the dorsal surfaces dealing with the UVC irradiation. rubrum in ex vivo porcine hoof culture at different depths, a cryostatic microtome was used.
Figure 3a shows a consultant periodic acid–Schiff -stained histology of a four-week porcine hoof tradition contaminated with T. rubrum invasion was demonstrated by quite a few hyphae branching out in each direction and subverting the nail structure.
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The frozen hoof fragments had been then rigorously sectioned at −25 °C, producing sections of 50-μm thickness. The 50-μm thick hoof sections had been inoculated on SDA plates and the time for colony outgrowth decided. This procedure was also carried out with complete infected hoof or toenail fragments after UVC irradiation. This allowed a semiquantitative assay for the inactivation fee primarily based on the beginning date of colony outgrowth on to SDA plates.